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KMID : 0620920120440020138
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Experimental & Molecular Medicine
2012 Volume.44 No. 2 p.138 ~ p.148
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Increased p190RhoGEF expression in activated B cells correlates with the induction of the plasma cell differentiation
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Ha Yun-Jung
Jeong Ji-Hye
Park Yu-Na
Lee Jong-Ran
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Abstract
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Previously, we demonstrated that the p190 Rho guanine nucleotide exchange factor (p190RhoGEF) was induced following CD40 stimulation of B cells. In this study, we examined whether p190RhoGEF and a downstream effector molecule RhoA are required for B cell differentiation. Expression of p190RhoGEF positively correlated with the expression of surface markers and transcriptional regulators that are characteristic of mature B cells with plasma cell (PC) phenotypes. Moreover, either the overexpression of p190RhoGEF or the expression of a constitutively active RhoA drove cellular differentiation toward PC phenotypes. B cell maturation was abrogated in cells that overexpressed p190RhoGEF and a dominant-negative form of RhoA simultaneously. CD40-mediated maturation events were also abrogated in cells that overexpressed either dominant-negative p190RhoGEF or RhoA. Together, these data provide evidence that p190RhoGEF signaling through RhoA in CD40-activated B cells drives the induction of the PC differentiation.
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KEYWORD
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antigens, CD40, ARHGAP35 protein, human, B-lymphocytes, CD40, cell differentiation, p190RhoGEF, plasma cells, rhoA GTP-binding protein
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